Wednesday, March 6, 2013

Principle of Chromatography

The technique of chromatography is based upon the fact that the different constituents of a mixture are absorbed to different extents by adsorbent.
Stationary Phase: is a thin layer of adsorbent coated on a plate
Mobile Phase: is a developing liquid which travels up the stationary phase, carrying the samples with it.
Solvent is a substance, especially a liquid that can dissolve another substance.
Common Solvent: Water, Ethyl alcohol, acetone, etc........
Adsorbent is a material which can easily absorb liquids.
Common Adsorbent: Filter paper(cellulose), Magnesium oxide, Silica gel, Alumina.

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